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Recombinant Enzyme Protein Digestion Carboxypeptidase B Proteomics Grade

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Shanghai Yaxin Biotechnology Co.,Ltd.
City: Shanghai
Province/State: Shanghai
Country/Region: China
Contact Person:Mr zhao

Recombinant Enzyme Protein Digestion Carboxypeptidase B Proteomics Grade

Brand Name : YaxinBio
Model Number : RCPB01
Certification : ISO 9001
Place of Origin : China
MOQ : 10mg
Payment Terms : T/T
Packaging Details : ice packaging
Price : 150$/10mg
Supply Ability : 1000g per week
Product : Recombinant Carboxypeptidase B
brand : YaxinBio
Protein content : 35% ~ 50%
Specific activity : ≥170 units/mg pro
Purity : > 95%
Cat. No. : RCPB01
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Recombinant Enzyme Protein Digestion Carboxypeptidase B Proteomics Grade


CAS: 9025-24-5
EC: 3.4.17.2

Source: Expressed in E. Coli.


Description


Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, the optimum pH is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is expressed in E.Coli and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation.


Main Features


SourceE. Coli
Purified byHPLC
FormatWhite or white-like or yellowish lyophilized
AdditivesTris salts, NaCl salts and carbohydrates
PuritySingle main band on SDS-PAGE
M.W.33.8kD
Contaminant activityLess than 10 ppm of recombinant trypsin.

Unit Definition:One Unit of carboxypeptidase B activity hydrolyzes one micromole of hippuryl-L-arginine per minute at 25℃, pH 7.65.


Advantages


Animal origin free:YaxinBio recombinant carboxypeptidase B belongs to the AOF level 3, eliminate the risk of virus presence, or of any other potential adventitious agents found in animal-derived carboxypeptitase B

Stability:A sterile recombinant carboxypeptidase B lyophilized eliminates the risk of contamination and decreases the chances of activity loss in the process of transport and storage.

High purity:

1) Recombinant carboxypeptidase B provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes with lower purity level.

2) No other contaminating proteases such as chymotrypsin and carboxypeptidase A.

3)Less than 10ppm of recombinant trypsin.


Recommend Usage


To prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.


Storage Instructions


Recommend recombinant carboxypeptidase B lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.



Product Tags:

serine protease

      

sequencing grade trypsin

      
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