Carboxypeptidase B for Enzymatic Hydrolysis of Protein Digesting
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids
lysine, arginine and histidine from the C-terminal end of
polypeptides. The molecular weight is 33.8kD, pI is 6.0 and the
optimum pH is 7.5-9.0. Carboxypeptidase B is competitively
inhibited by arginine and lysine. The enzyme is also inhibited by
metal chelating agents, e.g., EDTA. YaxinBio has cloned rat
carboxypeptidase B gene into E.coli for expression with equivalent properties compared to animal
original carboxypeptidase B .Recombinant carboxypeptidase B can
replace native carboxypeptidase B for using in a variety of
≥170 units/mg pro.
Single main band on SDS-PAGE
Less than 10 ppm of recombinant trypsin.
Unit Definition:One Unit of carboxypeptidase B activity hydrolyzes one micromole of
hippuryl-L-arginine per minute at 25℃, pH 7.65.
To prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM
Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000
(w/w), the optimum pH is pH 7-9.
STABILITY OF STORAGE AND TRANSPORT
Stability of storage: Recombinant carboxypeptidase B lyophilized
should be stored under 2-8℃ in sealed container.
It is stable within 24 months.After dissolved wth sterile water or 25mM Tris-HCl, it should be stored under -20℃.
It is no activity loss after 10 times repeated freezing and
Stability of transport: The product is stable by bue ice insulation transport.
Recommend recombinant carboxypeptidase B lyophilized should be
stored under 2℃-8℃ in sealed container. It is stable within 24
months.After dissolved, it should be stored under -20℃, It is
stable within 24 months and above 90% activity remained after 10
times repeated freezing and thawing.