Recombinant Carboxypeptidase B Proteomics Grade Enzyme with purity
200 u / mg Pro
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids
lysine, arginine and histidine
from the C-terminal end of polypeptides. The molecular weight is
33.8kD, the pH optimum
is 8.0, and pI is 6.0. Carboxypeptidase B is competitively
inhibited by arginine and lysine. The
enzyme is also inhibited by metal chelating agents, e.g., EDTA.
Recombinant Carboxypeptidase B is expressed in E.Coli and purified
by high pressure liquid chromatography. There is no trace of other
enzyme (such as carboxypeptidase A and chymotrypsin) activity. No
protease inhibitors such as
PMSF are present in the preparation.
Protein structure and sequence analysis, such as hydrolyze basic
amino acids lysine, arginine
and histidine from the C-terminal end of polypeptides.
Antibody quality control.
|Purity/Purification||Greater than 90% as determined by SDS-PA|
|Form/Format||The protein was lyophilized with 100mM NaCl and 20mM Tris pH-7.5.|
Sterile Filtered lyophilized powder.
|Unit Defenition||One Unit hydrolyzes one micromole of hippuryl-L-arginine per minute
at 25 degree C, pH-7.65.|
|Solubility||It is recommended to reconstitute the lyophilized Rat
Carboxypeptidase-B in sterile 18M Omega -cm H2O not less than 100
ug/ml, which can then be further diluted to other aqueous
|Biological Activity||200 units/mg protein.|
|Preparation and Storage||Recombinant Rat Carboxypeptidase-B although stable at room temp for
1 week, should be stored desiccated below -18 degree C. For long
term storage it is recommended to add a carrier protein (0.1% HSA
or BSA). Please prevent freeze-thaw cycles.|
|Other Notes for Cpb1 recombinant protein||Small volumes of Cpb1 recombinant protein may occasionally become entrapped in the seal of the product vial
during shipment and storage. If necessary, briefly centrifuge the
vial on a tabletop centrifuge to dislodge any liquid in the
container`s cap. Certain products may require to ship with dry ice
and additional dry ice fee may apply.|
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Prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM
Tris-HCl pH 7.65. The ratio
to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.
Recommend recombinant carboxypeptidase B lyophilized should be
stored under 2-8℃ in sealed container. It is stable within 24
months.After dissolved, it should be stored under -20℃, It is
within 24 months and no activity lose after 10 times repeated
freezing and thawing.