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Recombinant Carboxypeptidase B Proteomics Grade Enzyme with the purity 200 u /

Shanghai Yaxin Biotechnology Co.,Ltd.
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Shanghai Yaxin Biotechnology Co.,Ltd.
City: Shanghai
Province/State: Shanghai
Country/Region: China
Contact Person:Mr zhao

Recombinant Carboxypeptidase B Proteomics Grade Enzyme with the purity 200 u /

Brand Name : YaxinBio
Model Number : SRCPB01
Certification : ISO 9001
Place of Origin : China
MOQ : 1mg
Payment Terms : T/T
Packaging Details : dry ice
Price : 385$/mg
Supply Ability : 1000g per week
Delivery Time : five days
Product : Sequencing Grade Carboxypeptidase B
EC : 3.4.17.2
CAS : 9025-24-5
Specific activity : NLT 200 units/mg pro
Contaminant activity : No trypsin, chymotrypsin, carboxypeptidase A, or other proteases contaminant.
RELATED PRODUCT : Recombinant Trypsin (sequencing grade)
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Recombinant Carboxypeptidase B Proteomics Grade Enzyme with purity 200 u / mg Pro


Description


Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine

from the C-terminal end of polypeptides. The molecular weight is 33.8kD, the pH optimum

is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The

enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is expressed in E.Coli and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as

PMSF are present in the preparation.


Application


Protein structure and sequence analysis, such as hydrolyze basic amino acids lysine, arginine

and histidine from the C-terminal end of polypeptides.

Antibody quality control.


Main Features


Purity/PurificationGreater than 90% as determined by SDS-PA
GE.
Form/FormatThe protein was lyophilized with 100mM NaCl and 20mM Tris pH-7.5.
Sterile Filtered lyophilized powder.
Unit DefenitionOne Unit hydrolyzes one micromole of hippuryl-L-arginine per minute at 25 degree C, pH-7.65.
SolubilityIt is recommended to reconstitute the lyophilized Rat Carboxypeptidase-B in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity200 units/mg protein.
Preparation and StorageRecombinant Rat Carboxypeptidase-B although stable at room temp for 1 week, should be stored desiccated below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Other Notes for Cpb1 recombinant proteinSmall volumes of Cpb1 recombinant protein may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Recommend Usage

Prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio

to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.


Storage Instructions


Recommend recombinant carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable

within 24 months and no activity lose after 10 times repeated freezing and thawing.




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