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YaxinBio a Supplier of Recombinant Carboxypeptidase B expressed in E.coli

Shanghai Yaxin Biotechnology Co.,Ltd.
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Shanghai Yaxin Biotechnology Co.,Ltd.
City: Shanghai
Province/State: Shanghai
Country/Region: China
Contact Person:Mr zhao

YaxinBio a Supplier of Recombinant Carboxypeptidase B expressed in E.coli

Brand Name : YaxinBio
Model Number : RCPB01
Certification : NSF ISO 9001
Place of Origin : China
MOQ : 10mg
Payment Terms : T/T
Packaging Details : ice packaging
Price : 175$/100mg
Supply Ability : 100000g per year
Delivery Time : 7 work days
Product : Recombinant Carboxypeptidase B
Cat. No. : RCPB01
Specific activity : ≥170 u/mg pro
Physical form : White or white-like lyophilized powder
Additives : Tris salts, NaCl salts and carbohydrates
Protein content : 35% ~ 50%
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YaxinBio a Supplier of Recombinant Carboxypeptidase B expressed in E.coli


Recombinant Carboxypeptidase B


Description


Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, pI is 6.0 and the optimum pH is 7.5-9.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. YaxinBio has cloned rat carboxypeptidase B gene into E.coli for expression with equivalent properties compared to animal original carboxypeptidase B .Recombinant carboxypeptidase B can replace native carboxypeptidase B for using in a variety of biotechnological processes.

Main Features


Source

E. Coli

Purified by

HPLC

Purity

Single main band on SDS-PAGE

M.W.

33.8kD

Contaminant activity

Less than 10 ppm of recombinant trypsin.


Unit Definition


One Unit of carboxypeptidase B activity hydrolyzes one micromole of hippuryl-L-arginine per minute at 25℃, pH 7.65.

Applications
Carboxypeptidase B (EC 3.4.17.2, protaminase, pancreatic carboxypeptidase B, tissue carboxypeptidase B, peptidyl-L-lysine [L-arginine]hydrolase) is a carboxypeptidase that preferentially acts upon basic amino acids, such as arginine and lysine. This serum enzyme is also responsible for rapidly metabolizing the C5a protein into C5a des-Arg, with one less amino acid.

Recommend Usage


To prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.


Storage Instructions


Recommend recombinant carboxypeptidase B lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.

Pathway
Complement and Coagulation Cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Protein digestion and absorption, organism-specific biosystem
Function
carboxypeptidase activity;
metallocarboxypeptidase activity;
zinc ion binding

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