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Shanghai Yaxin Biotechnology Co.,Ltd.
Shanghai Yaxin Biotechnology Co.,Ltd.
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Carboxypeptidase Enzyme Animal Origin Free Carboxypeptidase B Proteomics Grade

Shanghai Yaxin Biotechnology Co.,Ltd.
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Carboxypeptidase Enzyme Animal Origin Free Carboxypeptidase B Proteomics Grade

Brand Name : YaxinBio
Model Number : SRCPB01
Certification : ISO 9001 2015
Place of Origin : China
MOQ : 10mg
Price : 150$/10mg
Payment Terms : T/T
Packaging Details : ice packaging
Supply Ability : 1000g per year
Delivery Time : 7 work days
Source : Recombinant E. Coli
Purified by : HPLC
Physical form : White or white-like lyophilized powder
Additives : Tris salts, NaCl salts and carbohydrates
Specific activity : ≥200 units/mg pro
Purity : > 95%
Contaminant activity : No chymotrypsin,carboxypeptidase A,and other protease contaminant. Less than 10 ppm of recombinant trypsin.
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Carboxypeptidase Enzyme Animal Origin Free Carboxypeptidase B Proteomics Grade


1.DESCRIPTION

Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, pI is 6.0 and the optimum pH is 7.5-9.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA.


2. MAIN FEATURES

SourceRecombinant E. coli
AppearanceWhite or off white, or yellowish powder
Specific activity≥200 units/mg pro.
SDS-PAGESingle major band
Molecular Weight(SDS-PAGE)33.8±3.4 kDa
Purity(HPLC)≥ 95%

3.RECOMMEND USAGE

Prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7.5-9.0.


4.STABILITY OF STORAGE AND TRANSPORT

Stability of storage: Sequencing grade carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container. It is stable within 24 months.After dissolved with sterile water or 25mM Tris-HCl, it should be stored under -20℃. Avoid repeated freezing and thawing.

Stability of transport: The product is stable by blue ice insulation transport.


5.PRODUCT ADVANTAGE

Sequence grade recombinant protein: high specificity,good stability.


Animal origin free: Sequencing grade carboxypeptidase B is no exogenous virus contamination,and any animal origin material is not used in the production process.


Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.


High purity: Higher specific activity.Host protein residues is less than the limits of biological products.


Lyophilized powder: The product is lyophilized powder and is easy to store and transport.


Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory

requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.


Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.


6.PRODUCT USAGE

1) Determination of protein C-terminal basic amino acids such as arginine, lysine and histidine.

2) detection of antibody basic peak.


Product Tags:

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