Carboxypeptidase Enzyme Animal Origin Free Carboxypeptidase B
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids
lysine, arginine and histidine from the C-terminal end of
polypeptides. The molecular weight is 33.8kD, pI is 6.0 and the
optimum pH is 7.5-9.0. Carboxypeptidase B is competitively
inhibited by arginine and lysine. The enzyme is also inhibited by
metal chelating agents, e.g., EDTA.
2. MAIN FEATURES
|Source||Recombinant E. coli|
|Appearance||White or off white, or yellowish powder|
|Specific activity||≥200 units/mg pro.|
|SDS-PAGE||Single major band|
|Molecular Weight(SDS-PAGE)||33.8±3.4 kDa|
Prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM
Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000
(w/w), the optimum pH is pH 7.5-9.0.
4.STABILITY OF STORAGE AND TRANSPORT
Stability of storage: Sequencing grade carboxypeptidase B
lyophilized should be stored under 2-8℃ in sealed container. It is
stable within 24 months.After dissolved with sterile water or 25mM
Tris-HCl, it should be stored under -20℃. Avoid repeated freezing
Stability of transport: The product is stable by blue ice
Sequence grade recombinant protein: high specificity,good
Animal origin free: Sequencing grade carboxypeptidase B is no
exogenous virus contamination,and any animal origin material is not
used in the production process.
Stable quality: Mass production can ensure stable and continuous
batch production.It is no difference between the batch and the
product quality is stable.
High purity: Higher specific activity.Host protein residues is less
than the limits of biological products.
Lyophilized powder: The product is lyophilized powder and is easy
to store and transport.
Compliance with regulatory requirements: Production equipment and
production environment comply with relevant regulatory
requirements, and the production process is in full compliance with
NSF ISO 9001: 2015 quality system and GMP guidelines.
Complete quality documents: we can provide relevant regular support
files in according to customers’ requirement.
1) Determination of protein C-terminal basic amino acids such as
arginine, lysine and histidine.
2) detection of antibody basic peak.