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Shanghai Yaxin Biotechnology Co.,Ltd.
Shanghai Yaxin Biotechnology Co.,Ltd.
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Animal Components Free Carboxypeptidase B For Arg-C And lys-C Sequencing Grade

Shanghai Yaxin Biotechnology Co.,Ltd.
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Shanghai Yaxin Biotechnology Co.,Ltd.
City: Shanghai
Province/State: Shanghai
Country/Region: China
Tel: 86-21-54336592
Contact Person:
Mr zhao

Animal Components Free Carboxypeptidase B For Arg-C And lys-C Sequencing Grade

Brand Name : YaxinBio
Model Number : SRCPB01
Certification : ISO 9001-2008
Place of Origin : China
MOQ : 100ug
Payment Terms : T/T
Packaging Details : ice packaging
Price : 198$/100ug
Supply Ability : 1000g per week
Product : Sequencing Grade Carboxypeptidase B
Manufacturer : YaxinBio
Source : E. Coli
Specific activity : NLT 200 units/mg pro
Contaminant activity : No trypsin, chymotrypsin, carboxypeptidase A, or other proteases contaminant.
RELATED PRODUCT : Sequencing Grade Chymotrypsin
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Animal Components Free Carboxypeptidase B For Arg-C And lys-C Sequencing Grade


Description

Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, the pH optimum is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is expressed in E.Coli and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation.


Main Features

Purity by HPLC

≥ 95%

Format

Lyophilized

Contaminant activity

No chymotrypsin, carboxypeptidase A, or other proteases contaminant.


RECOMMEND USAGE

Prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7.5-9.0.


Application

Protein structure and sequence analysis, such as hydrolyze basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides.

Antibody quality control.


Storage Instructions

Recommend recombinant carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and no activity lose after 10 times repeated freezing and thawing.


Email:Zhaozhi@yaxinbio.com


Product Tags:

sequencing grade modified trypsin

      

trypsin endopeptidase

      
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